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Chromatography resin for extraction, separation and purification of epothilone

Classification:
Separation and purification process development
Author:
Source:
Release time:
2019/09/24 17:22
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[Abstract]:
Epothilone (Epo) is a cytotoxic compound isolated from the fermentation broth of Sorangium cellulosum (Sorangium cellulosum), which has a biological activity similar to paclitaxel. Epothilone is a class of 16-carbon macrolide compounds, and a variety of biologically active analogues have been reported, such as Epothilone A (Epo A), Epothilone B (Epo B) and Epo Epo D (Epo D).

Chromatography resin for extraction, separation and purification of epothilone

Epothilone (Epo) is a cytotoxic compound isolated from the fermentation broth of Sorangium cellulosum (Sorangium cellulosum), which has a biological activity similar to paclitaxel. Epothilone is a class of 16-carbon macrolide compounds, and a variety of biologically active analogues have been reported, such as Epothilone A (Epo A), Epothilone B (Epo B) and Epo Epo D (Epo D). Epo has the advantages of good water solubility, simple structure, good chemical modification potential and high activity to tumor cells resistant to paclitaxel. It is a new anti-cancer drug with great market potential. The fermentation unit of epothilone is low, and the highest domestic unit is generally about 100mg/L, which puts forward higher requirements for the development of its separation and purification process. Jinan Bona Biotechnology Co., Ltd. has a high viscosity for the fermentation extract of epothilone. The structure of epothilone A (Epo A) and epothilone B (Epo B) are very similar. The SKP-10-4100 resin chromatography medium was developed to make the separation and purification process of epothilone simpler and the yield Can reach more than 70%.

The separation and purification process of epothilone is as follows: the fermentation broth and resin HZ816 are filtered through a sieve plate-the adsorption resin is collected-the purified water is washed until colorless-the resin is washed with 80% ethanol (A+B)-concentrated under reduced pressure ——Ethyl acetate extraction——Activated carbon decolorization——Concentration under reduced pressure and methanol dissolution——SKP-10-4100 resin chromatography——Epothilone B was obtained.

SKP-10-4100 chromatography resin is a new generation of chromatographic separation chromatographic media developed by Jinan Bona Biotechnology Co., Ltd. It has low chromatographic pressure and fast flow rate. It does not need a high-pressure preparation column. It can be packed with ordinary chromatography columns. Complete the perfect separation of epothilone, and the filler is one tenth of the price of silica-based modified filler.

If you purchase SKP-10-4100 chromatography packing or request samples, please contact our company, our company will provide you with detailed technical support.